Sample information

Collection date 04/14/2022
Captive / Cultivated? Captive / Cultivated
Group The Summit Country Day School

These are cultured fruit flies purchased by Carolina Biological and arrived on March 23, 2022. The strain was miniature apterous. 

Putative identification Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster


Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes


Wolbachia presence No
Confidence level High
Explanation of confidence level

We are confident in our controls. For our positive control showed the two genes being expressed while the negative control only had one gene, the COI. The water control also proved further our procedure was thorough for it did not have any genes.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Drosophila melanogaster was found to be negative for Wolbachia.
Report Inappropriate Post