Two-lined leather-winged beetle

April 27, 2022

Sample information
Picture
Photos by: Elly F.
Location
Collection date 04/05/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations
  • 6-9mm
  • orange, black, red
  • winged
  • pollinator
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

My confidence level is low because at many steps in this lab I was unsure of what I was doing.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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