Two-lined leather-winged beetle

Sample information

Photos by: Elly F.
Collection date 04/05/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
  • 6-9mm
  • orange, black, red
  • winged
  • pollinator
Putative identification Arthropoda Hexapoda Insecta Coleoptera


Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes


Wolbachia presence No
Confidence level Low
Explanation of confidence level

My confidence level is low because at many steps in this lab I was unsure of what I was doing.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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