Pill Bug

April 27, 2022

Sample information
Picture
Photos by: Carl S
Location
Collection date 04/13/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Oval shaped, with seven sets of legs and a hard outer shell, these creatures are best known for their ability to roll themselves into a perfectly shaped ball when threatened.
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The control was not clear so we are unsure about the results. Color of pill was brown.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

we aren’t sure because the control was unsure
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidae was found to be negative for Wolbachia.
Report Inappropriate Post