Six-Spotted Tiger Beatle – ADK-1

May 24, 2022

Sample information
Picture
Photos by: Xavier M.
Location
Collection date 05/02/2022
Captive / Cultivated? Captive / Cultivated
Group Woodford County High School
Observations

Collected using an alcohol vat in a malaise trap. Near a dried creek bed that occasionally floods, mature and juvenile trees are present. Some plastic litter. Medium vegetation, strong presence of honeysuckle. Surrounded by urban/suburban sprawl.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Carabidae Cicindela Cicindela sexguttata

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The beatle DNA displayed a purple band underneath it, indicating presence of wolbachia DNA.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Cicindela sexguttata was found to be postive for Wolbachia.
Report Inappropriate Post