Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/02/2022 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Woodford County High School |
| Observations | Collected using an alcohol vat in a malaise trap. Near a dried creek bed that occasionally floods, mature and juvenile trees are present. Some plastic litter. Medium vegetation, strong presence of honeysuckle. Surrounded by urban/suburban sprawl. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Carabidae Cicindela Cicindela sexguttata |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | The beatle DNA displayed a purple band underneath it, indicating presence of wolbachia DNA. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Cicindela sexguttata was found to be postive for Wolbachia. |
Water Boatman