Earwig – ADK2

May 24, 2022

Sample information
Picture
Photos by: Xavier M.
Location
Collection date 05/05/2022
Captive / Cultivated? Captive / Cultivated
Group Woodford County High School
Observations

Collected near a dry creek bed with medium vegetation, mature and young trees present. Suburban environment. Collected with a malaise trap.
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

There are two bands. The dark blue one indicates earwig DNA, and the purple indicates Wolbachia DNA.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be postive for Wolbachia.
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