Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/05/2022 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Woodford County High School |
| Observations | Collected near a dry creek bed with medium vegetation, mature and young trees present. Suburban environment. Collected with a malaise trap. |
| Putative identification | Arthropoda Hexapoda Insecta |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | There are two bands. The dark blue one indicates earwig DNA, and the purple indicates Wolbachia DNA.
|
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Insecta was found to be postive for Wolbachia. |
Water Boatman