Comb-Clawed Spider(Christopher m)

September 7, 2022

Sample information
Picture
Photos by: Christopher M
Location
Collection date 08/30/2022
Captive / Cultivated? Wild-caught
Group Antioch High School
Observations

Tiny

brown, speckled

by itself
Putative identification Arthropoda Chelicerata Arachnida Araneae

Methods
Extraction kit Wizard SV (Promega)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

The spider was an excellent choice for the lab when doing every step i could clearly see what was supposed to be happening. The liquids were murky but there wasn’t so much Debree that it got in the way of the lab and cause many problems. When following the lab i could see the visualization of what the lab is supposed to look like through the spider.

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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