Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/30/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Antioch High School |
| Observations | Tiny brown, speckled by itself |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae |
Methods |
|
| Extraction kit | Wizard SV (Promega) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | The spider was an excellent choice for the lab when doing every step i could clearly see what was supposed to be happening. The liquids were murky but there wasn’t so much Debree that it got in the way of the lab and cause many problems. When following the lab i could see the visualization of what the lab is supposed to look like through the spider. |
Results |
|
| Wolbachia presence | |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman