September 7, 2022

Sample information
Picture
Photos by: Ahmedullah Lodhi
Location
Collection date 08/30/2022
Captive / Cultivated? Wild-caught
Group Antioch High School
Observations

Pill bugs, 2 very big ones, 1 newborn. One was definitely a female as it was carrying a new born in its pouch. The newborn as well as the other Pill bug’s gender were unknown.
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae

Methods
Extraction kit Wizard SV (Promega)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

I trust my results, my naked eye simply cannot see them.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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