Mosquito

October 24, 2022

Sample information
Picture
Photos by: Samantha D.
Location
Collection date 09/20/2022
Captive / Cultivated? Captive / Cultivated
Group South Plainfield High School
Observations

The female larva was captured by Middlesex Mosquito Commission and grown to an adult size before given to our class.
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Culex

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

 

There was a strong band at the 500 mark.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culex was found to be postive for Wolbachia.
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