Sample information

Photos by: Akwesi A.
Collection date 09/20/2022
Captive / Cultivated? Captive / Cultivated
Group South Plainfield High School

Highly likely to be a female mosquito due to its similarities to other female mosquitoes. – Such as larger females.

Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Culex


Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Qiagen Blood or Tissue Kit with modification

P2 for ATL + N3 for AL


Wolbachia presence No
Confidence level Medium
Explanation of confidence level

There is a medium confidence level in the results due to the fact that after PCRing the DNA that would be used to find Wolbachia much of the DNA was lost in the PCR machine.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culex was found to be negative for Wolbachia.
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