Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/20/2022 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | South Plainfield High School |
| Observations | Highly likely to be a female mosquito due to its similarities to other female mosquitoes. – Such as larger females. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae Culex |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | Qiagen Blood or Tissue Kit with modification P2 for ATL + N3 for AL |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | There is a medium confidence level in the results due to the fact that after PCRing the DNA that would be used to find Wolbachia much of the DNA was lost in the PCR machine. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Culex was found to be negative for Wolbachia. |
Extraction and Analysis of Wolbachia from Halyomorpha halys
Tick
Roly-Poly
Idaho Beetle
Grammonota