Mosquito

September 20, 2022

Sample information
Picture
Photos by: Madison M.
Location
Collection date 09/20/2022
Captive / Cultivated? Captive / Cultivated
Group South Plainfield High School
Observations

Sex: Female
Larva Captured by Middlesex Mosquito Commission, grown to adults, donated to our class
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Culex

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain Other
Gel images
Protocol notes

The first image represents the Mosquito Gel. The second image represents the Wolbachia Gel.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Wolbachia had a strong band at 500
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culex was found to be postive for Wolbachia.
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