RA1 (red ants) Wolbachia Results

Sample information
Picture	Photos by: Ashley P
Location
Collection date	09/17/2022
Captive / Cultivated?	Wild-caught
Group	Hawaii Community College
Observations	Placed a couple pieces of dry cat food on the (screened-in) outdoor kitchen/ lanai floor. Within 24 hrs there were a group of very small red ants surrounding the piece of cat food. There were approximately 10-20 individuals. They moved relatively slowly and without any perceived organization. Within 24-48hrs they would leave that location. Under the dissecting microscope the small ants appeared to have lighter colored (almost see through) abdomens. With very long legs and antennae, as well as very large mandibles.
Putative identification	Arthropoda Hexapoda
Methods
Extraction kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system
Buffer	TBE
DNA stain	SYBR Safe
Gel images
Protocol notes	Research project planning DNA extraction PCR Gel electrophoresis
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	All controls worked, no 438bp Wolbachia bands present on the gel for this specimen sample.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Hexapoda was found to be negative for Wolbachia.

The Wolbachia Project Database