Lab Beetle

October 16, 2022

Sample information
Picture
Photos by: Danielle G.
Location
Collection date 09/21/2022
Captive / Cultivated? Wild-caught
Group Palm Beach Atlantic University
Observations

This beetle was caught dead outside of the Macarthur lab. It was stored in ethanol at 37 degrees Celsius, 3 hours before starting the experiment.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Geotrupidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Lane 1: 100Kb Ladder

Lane 2: Negative control

Lane 3: Positive Control

Lane 4: Other arthropod tested (Wasp)

Lane 5: Beetle tested

Lane 6: Water

Two arthropods were tested and are shown on the gel. DNA for the beetle, Coleoptera Geotrupidae is shown on lane 5. 6x loading dye was used for our samples, and ran at 100 V for 30 minutes.

 

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I have a very high confidence level due to the amplified band showing negative results for Wolbachia. The negative control presented with a single band on the gel and the same result appeared for the tested arthropod. The controls were amplified on the gel, negative control shows one band and positive control shows two bands.
Wolbachia 16S sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Geotrupidae was found to be negative for Wolbachia.
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