Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/21/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Palm Beach Atlantic University |
| Observations | This beetle was caught dead outside of the Macarthur lab. It was stored in ethanol at 37 degrees Celsius, 3 hours before starting the experiment. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Geotrupidae |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
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| Protocol notes | Lane 1: 100Kb Ladder Lane 2: Negative control Lane 3: Positive Control Lane 4: Other arthropod tested (Wasp) Lane 5: Beetle tested Lane 6: Water Two arthropods were tested and are shown on the gel. DNA for the beetle, Coleoptera Geotrupidae is shown on lane 5. 6x loading dye was used for our samples, and ran at 100 V for 30 minutes.
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Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I have a very high confidence level due to the amplified band showing negative results for Wolbachia. The negative control presented with a single band on the gel and the same result appeared for the tested arthropod. The controls were amplified on the gel, negative control shows one band and positive control shows two bands. |
| Wolbachia 16S sequence |
N/A
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence |
N/A
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Geotrupidae was found to be negative for Wolbachia. |
Water Boatman