Demacasp23-14 Orthoptera

Sample information
Picture	Photos by: Millie P
Location
Collection date	01/23/2023
Captive / Cultivated?	Wild-caught
Group	Georgia Southern University
Observations	Caught in the dead grass near the ponds at the Georgia Southern University Armstrong Campus. It was collected at approximately 1:15 on 1/23/23 using forceps. They were found hopping around in the grass and we used the forceps to pick them up and place them in the jar. It was partly cloudy and 59 degrees when we caught them.
Putative identification	Arthropoda Hexapoda Insecta Orthoptera
Methods
Extraction kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Other
Gel images
Protocol notes	DNA extraction kit of in house reagents was used.
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	According to my gel product, no bands were illuminated on the Wolbachia 16s rRNA for my specific DNA. The only band that was positive for Wolbachia was the positive control.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Orthoptera was found to be negative for Wolbachia.

The Wolbachia Project Database