Demacasp23-14 Orthoptera

April 10, 2023

Sample information
Picture
Photos by: Millie P
Location
Collection date 01/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Caught in the dead grass near the ponds at the Georgia Southern University Armstrong Campus. It was collected at approximately 1:15 on 1/23/23 using forceps. They were found hopping around in the grass and we used the forceps to pick them up and place them in the jar. It was partly cloudy and 59 degrees when we caught them.
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

According to my gel product, no bands were illuminated on the Wolbachia 16s rRNA for my specific DNA. The only band that was positive for Wolbachia was the positive control.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be negative for Wolbachia.
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