Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 01/23/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | It was sunny outside. It was 57 degrees Fahrenheit and I collected my insect in the tall grass by the entrance of the parking lot on the biology lab side on Armstrongs campus. I caught it on January 23, 2023 sometime in-between 1:00-1:30pm. I used the sweeping method to collect my insect |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | Our results were inconclusive so I’m not completely sure if its negative or not |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hemiptera was found to be negative for Wolbachia. |
Water Boatman