DEMACASp23-4JF Hemiptera

April 10, 2023

Sample information
Picture
Photos by: Joshua F
Location No map location data available.
Collection date 01/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

It was sunny outside.  It was 57 degrees Fahrenheit and I collected my insect in the tall grass by the entrance of the parking lot on the biology lab side on Armstrongs campus.  I caught it on January 23, 2023 sometime in-between 1:00-1:30pm.  I used the sweeping method to collect my insect
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Our results were inconclusive so I’m not completely sure if its negative or not
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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