Arthropod K25: Pseudoscorpion

April 27, 2023

Sample information
Picture
Photos by: Janelle P., Sydney Q.
Location
Collection date 04/06/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations

At a wooden picnic table on grassy land, underneath a tree. Found between the wooden planks of the table.

8 legs, orange/brown hue, pinchers observed, 0.1cm in length, light line vertically across abdomen, smooth body
Putative identification Arthropoda Chelicerata Arachnida Pseudoscorpiones

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We are confident that we have not found any trace of Wolbachia because all of our controls have correctly worked in our gel. Also we have seen that our other arthropod sample has been positive with wolbachia with a faint but clear line but not for the psuedoscorpion.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Pseudoscorpiones was found to be negative for Wolbachia.
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