Forficula Auricularia (ID: 2GH)

May 26, 2023

Sample information
Picture
Photos by: Sophia E.
Location
Collection date 04/06/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations
  • 12.7 mm- whole body
  • dark brown abdomen
  • 6 legs
Putative identification Arthropoda Hexapoda Insecta Dermaptera Forficulidae Forficula Forficula auricularia

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

(left- Arthropod gel, right- Wolbachia gel) We are not confident in our results due to errors such as mixing our sample tubes before loading them into the gel and errors in the DNA extraction process. We have bands in our arthropod gel in our lane for water which shows significant error. Additionally, only one of our Wolbachia lanes had bands (not including the ladder) when it should have had bands for a least two lanes, positive arthropod control and DNA control.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Forficula auricularia was found to be negative for Wolbachia.
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