American house spider (parasteaatoda tepidariorum) – 51846

May 26, 2023

Sample information
Picture
Photos by: Jenny Tran
Location
Collection date 04/05/2023
Captive / Cultivated? Captive / Cultivated
Group Arroyo High School
Observations

It was on a wall in a shady area, when you looked from far away the spider looked like it only had four legs. It was dark brown in color and moved very quickly as we appraoched it.
Putative identification Arthropoda Chelicerata Arachnida Araneae Theridiidae Parasteatoda Parasteatoda tepidariorum

Methods
Extraction kit none
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

We ran a regular PCR protcol twice but both times the data was faint and even our control was off. So our data was unreliable.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Our data didn’t show up, something along the way went wrong. We ran the data twice yet both times it did not come out the way we wanted. The tubes label was rubbed off so we don’t know the order we put things in.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Parasteatoda tepidariorum was found to be negative for Wolbachia.
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