Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/05/2023 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Arroyo High School |
| Observations | It was on a wall in a shady area, when you looked from far away the spider looked like it only had four legs. It was dark brown in color and moved very quickly as we appraoched it. |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Theridiidae Parasteatoda Parasteatoda tepidariorum |
Methods |
|
| Extraction kit | none |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | We ran a regular PCR protcol twice but both times the data was faint and even our control was off. So our data was unreliable. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | Our data didn’t show up, something along the way went wrong. We ran the data twice yet both times it did not come out the way we wanted. The tubes label was rubbed off so we don’t know the order we put things in. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Parasteatoda tepidariorum was found to be negative for Wolbachia. |
Water Boatman