Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/07/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Arroyo High School |
| Observations | -Under a piece of wood -Found near spiders and rollypolly -Damp dirt |
| Putative identification | Arthropoda Hexapoda Insecta Dermaptera Forficulidae Forficula Forficula auricularia |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Obtained by lifting a piece of wood in a damp, undisturbed area, picking up the arthropod, and placing it in a container. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Our wolbachia gel malfunctioned in some way that we aren’t able to get clear bands that will reveal whether or not our arthropod had wolbachia. None of our samples, even the DNA Ladder, were able to show. Our DNA seemingly collected at the top of our gel despite us putting it in the right orientation in the gel electrophoresis. We’re unsure what happened nor what our results are supposed to be. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman