T4P101234 (Pincherbug)

May 15, 2023

Sample information
Picture
Photos by: Angela M., Crystal N., Lynda T., Allen A.
Location
Collection date 04/07/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations

-Under a piece of wood

-Found near spiders and rollypolly

-Damp dirt
Putative identification Arthropoda Hexapoda Insecta Dermaptera Forficulidae Forficula Forficula auricularia

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Obtained by lifting a piece of wood in a damp, undisturbed area, picking up the arthropod, and placing it in a container.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

Our wolbachia gel malfunctioned in some way that we aren’t able to get clear bands that will reveal whether or not our arthropod had wolbachia. None of our samples, even the DNA Ladder, were able to show. Our DNA seemingly collected at the top of our gel despite us putting it in the right orientation in the gel electrophoresis. We’re unsure what happened nor what our results are supposed to be.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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