T4P101234 (Pincherbug)

Sample information
Picture	Photos by: Angela M., Crystal N., Lynda T., Allen A.
Location
Collection date	04/07/2023
Captive / Cultivated?	Wild-caught
Group	Arroyo High School
Observations	-Under a piece of wood -Found near spiders and rollypolly -Damp dirt
Putative identification	Arthropoda Hexapoda Insecta Dermaptera Forficulidae Forficula Forficula auricularia
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Rear half
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes	Obtained by lifting a piece of wood in a damp, undisturbed area, picking up the arthropod, and placing it in a container.
Results
Wolbachia presence	Unknown
Confidence level	Low
Explanation of confidence level	Our wolbachia gel malfunctioned in some way that we aren’t able to get clear bands that will reveal whether or not our arthropod had wolbachia. None of our samples, even the DNA Ladder, were able to show. Our DNA seemingly collected at the top of our gel despite us putting it in the right orientation in the gel electrophoresis. We’re unsure what happened nor what our results are supposed to be.
Wolbachia 16S sequence
Arthropod COI sequence
Summary

The Wolbachia Project Database