JJ22 – Crane Fly

May 15, 2023

Sample information
Picture
Photos by: Jolly J.
Location
Collection date 04/06/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations

found in grassy well lit area. flying around in a group with other crane flies
Putative identification Arthropoda Hexapoda Insecta Diptera Tipulidae

Methods
Extraction kit qiagen blood and tissue
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

There is low confidence because the gel was run two times and both times both the control and the samples were not visible. However, the ladder showed, but was very faint. With so many different possible sources of errors, maybe everything was not mixed properly or from human errors.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Tipulidae was found to be negative for Wolbachia.
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