Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/06/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Arroyo High School |
| Observations | found in grassy well lit area. flying around in a group with other crane flies |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Tipulidae |
Methods |
|
| Extraction kit | qiagen blood and tissue |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | There is low confidence because the gel was run two times and both times both the control and the samples were not visible. However, the ladder showed, but was very faint. With so many different possible sources of errors, maybe everything was not mixed properly or from human errors. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Tipulidae was found to be negative for Wolbachia. |
Water Boatman