Thrive Scholars – Summer Academy – Pillbug – TATT

August 9, 2023

Sample information
Picture
Location
Collection date 08/08/2023
Captive / Cultivated? Wild-caught
Group Thrive Scholars
Observations

Dark gray pillbug.
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

We followed the protocol on the website. The top gel is the Arthopod gel and the bottom is the Wolbachia gel.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

In the image, the majority of the DNA BPs were smeared meaning that it was difficult to not only determine the BP of the different sections but it was also difficult to see clear bands.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidium vulgare was found to be negative for Wolbachia.
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