Thrive Scholars – Cicada Killer Wasp – “Butterfly-Wasps”

August 9, 2023

Sample information
Picture
Photos by: Mauricio C.
Location
Collection date 08/07/2023
Captive / Cultivated? Wild-caught
Group Thrive Scholars
Observations
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Crabronidae Sphecius Sphecius speciosus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

We followed website protocol.

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

The top lanes contained arthropod primers; the bottom lanes contained Wolbachia primers. For our first sample of insect DNA with Wolbachia primers (the 2nd bottom lane), there was an occurrence of DNA. Though, it is smudged and not perfectly accurate.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Sphecius speciosus was found to be postive for Wolbachia.
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