Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/07/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Thrive Scholars |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Crabronidae Sphecius Sphecius speciosus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | We followed website protocol. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level |
The top lanes contained arthropod primers; the bottom lanes contained Wolbachia primers. For our first sample of insect DNA with Wolbachia primers (the 2nd bottom lane), there was an occurrence of DNA. Though, it is smudged and not perfectly accurate. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Sphecius speciosus was found to be postive for Wolbachia. |
Water Boatman