Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/11/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Antioch High School |
| Observations | My arthropod has yellow belly with a black back it has long black antennas and a red and black head. It was found outside in the grass in the back of the school in between the cracks of the grass. |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera Gryllidae Acheta Acheta domesticus |
Methods |
|
| Extraction kit | promega wizard SV |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | We may have accidently washed our DNA out. nothing showed up Confident we did this wrong |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | This is my level because we might have accidently washed out the DNA so it might have gone wrong. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman