Cricket

October 4, 2023

Sample information
Picture
Photos by: Linette M
Location
Collection date 09/11/2023
Captive / Cultivated? Wild-caught
Group Antioch High School
Observations

My arthropod has yellow belly with a black back it has long black antennas and a red and black head. It was found outside in the grass in the back of the school in between the cracks of the grass.
Putative identification Arthropoda Hexapoda Insecta Orthoptera Gryllidae Acheta Acheta domesticus

Methods
Extraction kit promega wizard SV
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

We may have accidently washed our DNA out.

nothing showed up

Confident we did this wrong

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

This is my level because we might have accidently washed out the DNA so it might have gone wrong.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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