Calligrapha Serpentina

October 4, 2023

Sample information
Picture
Photos by: Basil F
Location
Collection date 09/11/2023
Captive / Cultivated? Wild-caught
Group Antioch High School
Observations

Collected from school garden box nestled between the leaves of a plants thick stem. Black with yellow spots and a hard outer shell. 72 degrees, terrestrial habitat, found during fall. Found with 3 other bugs bunched together.
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit promega wizard SV
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

We may have contaminated the pipette tip. Our results were very flawed since we didn’t have the arthropod in the electro freeze long enough. None of the arthropods were showed to be infected with Wolbachia. My confidence is very low.

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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