Snow Mosquito

December 15, 2023

Sample information
Picture
Photos by: Cameron M.
Location
Collection date 09/20/2023
Captive / Cultivated? Wild-caught
Group Scarborough High School
Observations

Found outside behind Scarborough High School in the back parking lot. Captured while clinging onto hair. The temperature was 72 degrees. The season was fall. Small (5mm – 6mm), six legs, brown and black.
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

High confidence level. Sample two. Both positive and negative controls successfully worked. Supports evidence that arthropod is Wolbachia positive.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be postive for Wolbachia.
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