Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/20/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Scarborough High School |
| Observations | Found outside behind Scarborough High School in the back parking lot. Captured while clinging onto hair. The temperature was 72 degrees. The season was fall. Small (5mm – 6mm), six legs, brown and black. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | |
| Single or Duplex PCR | |
| Gel electrophoresis system | |
| Buffer | |
| DNA stain | |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level |
High confidence level. Sample two. Both positive and negative controls successfully worked. Supports evidence that arthropod is Wolbachia positive. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diptera was found to be postive for Wolbachia. |
Water Boatman