Sample information

Photos by: Madison R
Collection date 09/20/2023
Captive / Cultivated? Wild-caught
Group Scarborough High School

Found near a large tree on school grounds in a suburban community in early fall. It was about 70 degrees. It was a larger black ant and had 6 legs.

Putative identification Arthropoda Hexapoda Insecta Hymenoptera


Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes


Wolbachia presence Yes
Confidence level High
Explanation of confidence level

All controls showed the expected results, no known errors.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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