Crane Fly

Sample information

Photos by: Jacob M
Collection date 09/20/2023
Captive / Cultivated? Wild-caught
Group Scarborough High School

Slender body with six long legs, brownish in color, wings.

Putative identification Arthropoda Hexapoda Insecta Diptera Tipulidae


Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes


Wolbachia presence No
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Tipulidae was found to be negative for Wolbachia.
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