Sample information
Picture	Photos by: Jacob M
Location
Collection date	09/20/2023
Captive / Cultivated?	Wild-caught
Group	Scarborough High School
Observations	Slender body with six long legs, brownish in color, wings.
Putative identification	Arthropoda Hexapoda Insecta Diptera Tipulidae
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Tipulidae was found to be negative for Wolbachia.