Crane Fly

January 9, 2024

Sample information
Picture
Photos by: Jacob M
Location
Collection date 09/20/2023
Captive / Cultivated? Wild-caught
Group Scarborough High School
Observations

Slender body with six long legs, brownish in color, wings.
Putative identification Arthropoda Hexapoda Insecta Diptera Tipulidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Tipulidae was found to be negative for Wolbachia.
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