Flagge Arda und Nishant 01

October 24, 2024

Sample information
Picture
Photos by: Nishant S.
Location
Collection date 08/28/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

Im Spinnennetz im Keller eines Gebäudes. Trockenluft.
Putative identification Arthropoda Chelicerata Arachnida Araneae Theridiidae

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

1. Vortex und Zentrifuge verwechselt
2. Zu kurz gevortext
3. Falsch zentrifugiert

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Geltest war Wolbachia negativ
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Theridiidae was found to be negative for Wolbachia.
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