Sample information

Collected during summer, found on the sidewalk in front of house, it was 69 degrees Fahrenheit outside, urban habitat, the arthropod is bright green, has one body segment, black and white antennae, and is and one centimeter long.

Methods

DNA Extraction:

Arthropod was difficult to crush up as the outer hard shell was still attached.

PCR: No mistakes made

Gel Electrophoresis, Arthropod CO1:

The first lane of our Gel #1 contained our DNA ladder, the second lane contained our A1 sample (Luna Moth), third lane had A2 sample (European Hornet), fourth lane had sample A3 (Green Stink Bug), Lanes 5 through seven contained our positive arthropod control, our negative arthropod control and our H2O control.

Our controls worked so our results are valid.

Gel Electrophoresis, Wolbachia:

In our first gel, we had in lane one our DNA ladder, lane 2 had sample A1 (Luna Moth), the third lane had sample A2 (European Hornet), our fourth lane had sample A3 (Green Stink Bug), and then in lanes 5 through 7 had our positive Wolbachia control, negative Wolbachia control, and our H2O control. Our controls worked which means we experienced no contamination and our results are valid.

Results

We are very confident in the results since each control used did exactly what they were supposed to and we experienced NO contamination or errors.