Long Centipede (Strigamia bothriopus)

April 2, 2025

Sample information
Picture
Photos by: Pranav D.
Location
Collection date 03/10/2025
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

The arthropod looks yellowish-orange, and has a lot of legs coming out of the sides of its body. Its antennae are thicker than its legs, and at the front it wields a pair of two sharp looking fangs/pinchers.
Putative identification Arthropoda Myriapoda Chilopoda Geophilomorpha Linotaeniidae Strigamia

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Since the arthropod and Wolbachia controls came out as expected, that means that no contamination occurred after the extraction process. Furthermore, in addition to gel electrophoresis, we also quantified our DNA using a NanoDrop spectrophotometer, and the results came back in ranges that showed no signs of excessive contamination or inefficient reagents (e.g. excessive protein absorbance from ineffective proteinase K).
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Strigamia was found to be negative for Wolbachia.
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