Camponotus pennsylvanicus

June 6, 2025

Sample information
Picture
Photos by: Michael C.
Location
Collection date 06/03/2025
Captive / Cultivated? Wild-caught
Group Pingry School
Observations

The insect was found crawling up a tree in the woods behind the Pingry School. The insect had a black-colored body, three segments, six legs, and two antennae
Putative identification Arthropoda Insecta Hymenoptera Formicidae Camponotus Camponotus pennsylvanicus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes
  1. Cell lysis and Precipitation
  2. DNA purification
  3. DNA elution
  4. PCR
  5. Gel Electrophoresis

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I observed bands in both the Arthropod sample lane and the Wolbachia sample lane, indicating that the target DNA was amplified. Furthermore, the sequencing results showed a 100% match for both the Arthropod and Wolbachia sequences.
Wolbachia 16S sequence Download FASTA    Download AB1
Arthropod COI sequence Download FASTA    Download AB1
Summary The Camponotus pennsylvanicus was found to be postive for Wolbachia.
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