Sample information

I caught this hornet in my second floor bathroom. The season was early fall/late summer. The temperature was about 65 degrees F. This is typically a terrestrial organism but was in my home. Color: Black and Yellow,  6 legs, 1 pair of wings,
antennae length of head,  eggshaped abdomen
sharp pinsers.

Methods

PCR

DNA was not properly included during this process. This resulted in a blank gel electrophoresis.

Gel electrophoresis, Arthropod CO1

Lane one contained DNA Ladder, lane two contained A-1 (the hornet), lane 3 contained A-2, these were the only two that did not show up well. I believe the gel electrophoresis reflected an issue regarding the PCR process, the DNA did not show up well due to a lack of DNA in the PCR. Everything else worked well.

Gel electrophoresis, Wolbachia

Lane one contained DNA Ladder, lane two contained A-1 (the hornet), lane 3 contained A-2, lane 3 contained A-3, lane 4 contained A-4, lane 5 contained Wolbachia positive DNA, lane 6 contained Wolbachia negative DNA, lane 7 contained water. Each control worked and outside of A-1 and A-2, no mistakes appeared to have been made.

Results

I a confident that this arthropod is does not have Wolbachia. My thought process being that this PCR along with A-2 were repeated due to a mistake. Since two processes were made to determine they were negative that boosts my confidence further