Fruit Fly GRP15

May 22, 2026

Sample information
Picture
Entry by: (No pictures were taken of the arthropod outside of the collection tube)
Location
Collection date 04/20/2026
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

The fruit Fly was caught in a temperate environment at 11:42am. It was sunny and 70 degrees Fahrenheit. The fly was found on a bush in the shade.
Putative identification Arthropoda Insecta Hymenoptera Halictidae Lasioglossum

Methods
Extraction kit QIAGEN DNeasy kit
DNA extraction location
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer 1X TAE
DNA stain uv
Gel images
Protocol notes

DNA Extraction: The entire body of the fly was crushed because its body was around 2 millimeters in length

Gel Eletrophoresis Lanes for CO1 arthropod and Wspec Wolbachia:

1- DNA Ladder

2- Pill bug (sample)

3- Fly (sample)

4- Millipede (sample)

5- DNA (+)

6- Wolbachia (+) control

7- Wolbachia (-) control

8- Water

Analysis: The fruit fly had two bands on the gel that are suspected to be both an arthropod band and a Wolbachia band. However, the Wolbachia positive control only produced one band, so the results are not definitive.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The controls for the gel run were not successful, as the Wolbachia positive control should have produced 2 bands (one for Wolbachia and one for an arthropod), but only one band was visible on the gel. Therefore, even though the fly sample produced two bands, it is not conclusive whether one is an arthropod band and the other is a Wolbachia band.
Wolbachia 16S sequence Download FASTA    Download AB1
GGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TGATCAGGTATAATTGGAGCCTCATTAAGTATAATTATTCGAATAGAATTAAGTGCTCCTGGAAAATGAATTAATAATGATCAAATTTATAATACTATTATTACTTCACATGCATTCGTAATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGATTTGGAAATTGATTAGTCCCTTTAATAATTGGAGCACCTGATATAGCATTCCCTCGAATAAATAATATAAGATTTTGATTACTTATTCCATCTATATTTATATTATTAATAAGAAGAATTATATCTTCTGGTTCAGGAACTGGATGAACTATTTATCCACCATTATCTTCAATTATATATCATTCATCTATTTCTGTAGATTATACTATTTTTTCATTACATATTGCAGGAATTTCATCTATTATAGGAGCAATTAATTTTATTGTATCTATTTTATTAATAAAAAATATTTCAATTAATTATGATCAAATCCCTTTATTCCCATGATCAGTAAAAATTACCGCTATTTTATTATTATTATCTTTACCAGTACTAGCAGGAGCAATTACTATACTTTTAACAGATCGAAATTTAAATACTTCTTTTTTTGACCCTTCAGGAGGAGGAGACCCAATTCTTTATCAACATTTATTTTGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lasioglossum was found to be postive for Wolbachia.
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