CHIUAP

November 3, 2026

Sample information
Picture
Location
Approximate location: 31.97, -81.16
Collection date
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Data Collection

  • Collected: 09/01/2026
  • Time of day: 10:20AM
  • Location: Found in the northwest and western area outside of the Science Center.
  • Habitat: Small trees, bushes, and underneath rocks.
  • Collection Methods: Sweep net, beat net, and tray method.
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Followed the proper testing procedures to obtain valid results.
Wolbachia 16S sequence
Arthropod COI sequence
GGGCAGGNNTAGTAGGNACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAG AGGAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAA TACCTATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTA AATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAAC AGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTAC ATTTAGCANGAATTTCCTCAATTTTANGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGNATAATA CNTGATAAAACTCCNTTATTTGTATGATCTGTNCTTAT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
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