CHI-EAM Orthoptera

November 3, 2026

Sample information
Picture
Entry by: ESMERALDA MARCELIN
Location
Approximate location: 31.97, -81.16
Collection date 09/02/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Large green field approximate to a greenhouse.
Putative identification Arthropoda Insecta

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA Extraction kit in house was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I genuinely thought that it would be a hit or miss on whether my insect would have wolbachia
Wolbachia 16S sequence
Arthropod COI sequence
CGGGGNNAGTAGGAACCTCAATAAGATTATTAATTCGAGCTGAGCTAGGACA ACCAGGTTTTCTTATTGGAGATGATCAAATTTATAACGTTATTGTAACGGCACATGCCTTTGTTATAATTTTTTTTATAG TTATACCAATTATAATCGGAGGATTCGGAAATTGGTTAGTTCCTTTAATATTGGGGGCTCCAGATATAGCTTTTCCACGA ATAAACAATATAAGCTTTTGACTTTTACCCCCATCCTTGACCTTACTTCTAGCCAGAAGTCTTGTCGAAAACGGAGCAGG TACTGGTTGAACAGTTTATCCACCACTTTCTGCTAATATTGCGCATGCAGGAGCCTCAGTTGATCTAGCTATCTTCTCAT TACATTTAGCAGGTATTTCCTCAATTTTAGGGGCTGTAAATTTTATTACTACAACAATTAATATACGAGCCCCTGGAATA TCCCTAGATCAAACCCCCTTATTTGTATGAGCAGTAGCTATCACAGCACTACTTTTATTATTATCTTTACCAGTATTAGC AGGAGCTATTACTATATTATTAACCGATCGAAATTTAAATACATCCTTCTTTGACCCTGCTGGANGTGGAGACCCTATTC TTTACCAACACTTATTCTGATTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be negative for Wolbachia.
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