NESACKSp23-13-AD Blattodea

April 13, 2023

Sample information
Picture
Photos by: Andrew D
Location
Collection date 01/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Date of Collection- Thursday 1/26/23

Time of Collection- 10:12 AM

Location of Collection- Northeast side of pond of the Georgia Southern Armstrong campus

Address: 11935 Abercorn St, Savannah, GA 31419

Environment (habitat) of Collection- Windy, Cold temp. (490F), Edge of muddy bank with algae floating in the shaded water

Methods of Collection- 

  • Beat Stick Method: Hit branches and leaves to collect falling ladybugs and other insects on a rectangular cloth-net
  • D-net method: Used for aquatic collecting by swirling net in a circular motion of krill-like organisms
  • Post-lab: Soft forces to grab microorganisms and inserted into small vials to transport/hold when in classroom

 
Putative identification Arthropoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Our positive and negative controls each had 1 band, no template control had no band. Wolbachia had 2 bands and the my 1 band for my partner and I’s insect orders.
Wolbachia 16S sequence
TATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTcccacttcAtG CACTCGAGTTGCAGAGTACAATCCGAACTGAgaccttttttaaGGGATTA GCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTG TGTAGCCcactccataAGGGCCATGATGACTTGACATCATCCCCACCTTC CTCCAGTTTATCACTAGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATG GTAACTAAGGATGAGGGTTACGCTCGTTGCGGGACTTAACCCAACATCTc ACGACACgAGCtgataacAGCCATGCAACACCTGTgtgAAATCCGG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary The Blattodea was found to be postive for Wolbachia.
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