Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/11/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Antioch High School |
| Observations | It was on a tree, and I put my tube on it, and it came into the tube itself. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
|
| Extraction kit | promega wizard SV |
| DNA extraction location | |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | We made mistake in process of PCR. I think it was contaminated and thus we did not get the exact results. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | Confidence level is low because our PCR tests were not what we expected. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hemiptera was found to be negative for Wolbachia. |
Water Boatman