October 4, 2023

Sample information
Picture
Photos by: Dhruvi Patel
Location
Collection date 09/11/2023
Captive / Cultivated? Wild-caught
Group Antioch High School
Observations

It was on a tree, and I put my tube on it, and it came into the tube itself.
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit promega wizard SV
DNA extraction location
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

We made mistake in process of PCR. I think it was contaminated and thus we did not get the exact results.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Confidence level is low because our PCR tests were not what we expected.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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