October 4, 2023

Sample information
Picture
Photos by: dhruva patel
Location
Collection date 09/11/2023
Captive / Cultivated? Wild-caught
Group Antioch High School
Observations

I saw it on grass and wall I collected from ground because it’s easy it looks like large beetle, ridges on its elytra wing. coverings small metallic ground Bettles .
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit promega wizard SV
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

i think so we did PCR wrong because when we see the result, we didn’t see good result. i Guss we put wrong amount of CNA in the gel .

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

my confidence level is low . because we did not see good result after this all
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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