Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/11/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Antioch High School |
| Observations | I saw it on grass and wall I collected from ground because it’s easy it looks like large beetle, ridges on its elytra wing. coverings small metallic ground Bettles . |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | promega wizard SV |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | i think so we did PCR wrong because when we see the result, we didn’t see good result. i Guss we put wrong amount of CNA in the gel . |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | my confidence level is low . because we did not see good result after this all |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman