|Captive / Cultivated?||Captive / Cultivated|
|Group||Palm Beach Atlantic University|
I collected only one cricket from a container containing many other crickets. The crickets were living in an environment with mulch/straw. I collected this cricket from PetSmart in West Palm Beach, FL 33401 on September 7, 2023 at 3:00pm.
|Putative identification||Arthropoda Hexapoda Insecta Orthoptera Gryllidae|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Partial abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||Standard electrophoresis system|
DNA Extraction: I removed the exoskeleton and only dissected a piece of the lower abdomen out. I made sure to crush the cricket abdomen really well.
|Explanation of confidence level||
All of my controls worked as expected. I did not have any problems with the protocol. The bands appeared as they should have; therefore, I am confident my arthropod is not infected with Wolbachia.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Gryllidae was found to be negative for Wolbachia.|