Ant

October 23, 2023

Sample information
Picture
Photos by: Brandy R., Nikki S.
Location
Collection date 09/19/2023
Captive / Cultivated? Wild-caught
Group Palm Beach Atlantic University
Observations

On a hot, sunny day, the ant was collected from outside on a tree late afternoon in West Palm Beach, Florida. The cricket was store-bought from Boynton Beach, Florida in a cage that resembled its natural habitat.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Gel Electrophoresis: Since the ant was small, I used the whole body for DNA extraction.

Lanes for Gel:

-Lane 1: 1 kb ladder

-Lane 2: DNA from ant

-Lane 3: DNA from cricket

-Lane 4: Arthropod positive control (Drosophila with Wolbachia)

-Lane 5: Arthropod negative control (Drosophila without Wolbachia)

-Lane 6: Wolbachia DNA

-Lane 7: Water

Analysis: The top band should be present in all lanes except Arthropod (-) control and water. The bottom band indicates Wolbachia is present as seen in the Arthropod (+) control, Wolbachia DNA, and ant DNA.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

My band for ant DNA extraction is very faint on the actual gel and harder to see in the picture but the band for Wolbachia in the ant is present. DNA extraction should be done properly to get a more prominent top band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Formicidae was found to be postive for Wolbachia.
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