Sample information

Photos by: Lourie A
Collection date 09/09/2023
Captive / Cultivated? Wild-caught
Group Palm Beach Atlantic University

Arthropod was found in a grassy area in a field of a park in Florida during a sunny morning.

Putative identification Arthropoda Hexapoda Insecta Odonata


Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

DNA Extraction: The abdomen of the damselfly was cut from its body then crushed really well in order to extract its DNA.

Gel electrophoresis Lanes:

The wells of the gel were loaded in the following order (assuming lanes are shown on the left in the picture)

8- empty

7- Water (negative control)

6- + DNA control PCR control

5- -Arthropod PCR/ DNA extraction control

4- +Arthropod DNA extraction control

3- damselfly

2- cricket

1- 1 kb plus DNA ladder


There were bands in all lanes except water confirming that our DNA extraction and PCR techniques worked as they should. All of the controls also showed their expected bands.


Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Im very confident that my results are accurate. All of my controls worked as expected and the protocol was followed very closely. We ensured to work efficiently and carefully to avoid any cross contamination that could’ve occurred.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Odonata was found to be postive for Wolbachia.
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