November 7, 2023

Sample information
Picture
Location
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Found in dirt by lane liberty on Armstrong campus.

The weather was partly cloudy.

The collection method used was the tray method.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in house regents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I have high confidence because my gel showed that my insect had no Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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