Horned Passalus Beetle

November 7, 2023

Sample information
Picture
Photos by: Brithany N.
Location
Collection date 11/01/2023
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations
  • Beetle was found under a fallen rotting log in Long Hunter State Park.
  • Found with 4 other beetles
  • Beetle was approximately 3 cm long
  • Collected in the morning around 10 or 11
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

DNA Extraction: I dissected the abdomen out, and did not include the exoskeleton.

Gel Electrophoresis lanes:

  1. DNA Ladder
  2. Arthropod 1
  3. Arthropod 2
  4. Arthropod 3 (mine)
  5. + Arthropod control
  6. – Arthropod control
  7. + DNA control
  8. Water

Analysis: My controls worked, but my sample did not have a band at all. I believe the problem occurred in the DNA extraction lab.

 

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

I am confident that the presence for Wolbachia in my arthropod is unknown because the DNA control is missing in the first 3 cells meaning no DNA was exposed. What this proves is that during the DNA extraction lab the sample wasn’t grinded all the way to expose high DNA.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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