LOGACEF23-CB, Hemiptera

November 7, 2023

Sample information
Picture
Photos by: Chandler B.
Location
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Eastern Standard Time: 9:40 AM

Weather Conditions: 85 degrees Fahrenheit, sunny and 74% humidity.

Habitat: Field of tall grass

Collection Method: Sweeping net and stored in clear, plastic jar.
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

I am highly confident that my results are undetermined/unknown. In the gel image provided above, the Wolbachia band is shown however, the common CO1 Insect gene is absent. Cross contamination or an occurrence during DNA extraction could be reasons why my conclusion is unclear.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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