BroACH-F23CHP Dermaptera

Sample information
Picture	Photos by: Carmen P.
Location
Collection date	08/23/2023
Captive / Cultivated?	Wild-caught
Group	Georgia Southern University
Observations	Time of collection: Approx. 1:30pm – 2pm Location: North End of Georgia Southern Armstrong Campus Environment of Collection: Moist Tall Grass in Humid Weather Method of Collection: Sweep Net
Putative identification	Arthropoda Hexapoda Insecta Dermaptera
Methods
Extraction kit
DNA extraction location	Partial abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Other
Gel images
Protocol notes	DNA extraction kit of in-house reagents was used.
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	I am confident in my results due to my controls displaying the correct amount of DNA bands. My positive control had two band, my negative control had one band, and my no template control has zero bands. I did notice the insect CO1 gene in my PCR results, however the low exposure in the picture makes it not visible.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Dermaptera was found to be negative for Wolbachia.

The Wolbachia Project Database