LOGACEF23-LN Orthoptera

November 9, 2023

Sample information
Picture
Photos by: Lauren N.
Location
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Time: 6:32 p.m.

Location: Grassy area under pine trees

Collection method: Collection jar

Temperature/weather: 82 degrees F, partly cloudy, slight breeze
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

My results came back as undetermined, so I cannot be very confident in them since I am unable to compare them to the results of other insects in Orthoptera.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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