Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/22/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Time: 6:32 p.m. Location: Grassy area under pine trees Collection method: Collection jar Temperature/weather: 82 degrees F, partly cloudy, slight breeze |
| Putative identification | Arthropoda Insecta Orthoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | My results came back as undetermined, so I cannot be very confident in them since I am unable to compare them to the results of other insects in Orthoptera. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica