Limoniidae

January 9, 2024

Sample information
Picture
Photos by: Nina W., Johanna D.
Location
Collection date 11/09/2023
Captive / Cultivated? Wild-caught
Group Gymnasium Immensee
Observations

The weather ranged at around 10 degrees Celsius. It had just stopped raining so it was cloudy and humid. The insect was found in the air.
Putative identification Arthropoda Hexapoda Insecta Diptera Limoniidae

Methods
Extraction kit Edwards Buffer
DNA extraction location body without wings
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

The circled result is the result for Limoniidae.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

As you can see the circled result is not very clear in the added image file. Therefore we are not fully sure if it can be read as a result or if there was a problem with the gel electrophoresis.

 

 

 
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Limoniidae was found to be negative for Wolbachia.
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