Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/09/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Gymnasium Immensee |
| Observations | The weather ranged at around 10 degrees Celsius. It had just stopped raining so it was cloudy and humid. The insect was found in the air. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Limoniidae |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | body without wings |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The circled result is the result for Limoniidae. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | As you can see the circled result is not very clear in the added image file. Therefore we are not fully sure if it can be read as a result or if there was a problem with the gel electrophoresis.
|
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Limoniidae was found to be negative for Wolbachia. |
Water Boatman