Spaherium corneun

Sample information

Picture
Location
Collection date 11/13/2023
Captive / Cultivated?
Group Walton High School
Observations
Putative identification Mollusca Bivalvia Sphaeriida Sphaeriidae Pisidium Pisidium moitessierianum

Methods

Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Gonads dissected
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Translucent white clam found underneath rubble in shallow murky water.

We tested the Spaherium corneun for the Wolbachia barcoding and the Arthropod Barcoding gene.

Results

Wolbachia presence No
Confidence level Medium
Explanation of confidence level

In our experiment, we have low confidence that the Spaherium corneun is not Wolbachia positive.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Pisidium moitessierianum was found to be negative for Wolbachia.
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