Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/13/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Pea shaped clam with a thick striated shell. Found in muddy creekwater. Pearl-like color with brown splotches. |
| Putative identification | Mollusca Bivalvia Sphaeriida Sphaeriidae Pisidium Pisidium moitessierianum |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Gonads dissected |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Translucent white clam found underneath rubble in shallow murky water. We tested the Spaherium corneun for the Wolbachia barcoding and the Arthropod Barcoding gene. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | In our experiment, we have low confidence that the specimen is not Wolbachia positive but it cannot be determined because our gel electrophoresis results failed and were untrustworthy. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1
Oniscus asellus GC1
Oniscus Asellus (LS1)