Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Date of collection: 1/23/24 Time of collection:1000hrs Location of collection: West side of Georgia Southern University (Savannah) Environment (habitat) of collection: Vegetation and under logs Method of collection: Sweep net and digging in the dirt |
| Putative identification | Arthropoda Hexapoda Insecta Blattodea |
Methods |
|
| Extraction kit | |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I am confident that the controls work. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Blattodea was found to be negative for Wolbachia. |
Water Boatman