Sample information

Photos by: Brianna K
Collection date 01/23/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University

-under old debris laying on ground

-dirt and sand, damp, dark, place with wood pieces

Putative identification Arthropoda Hexapoda Insecta Isoptera


Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.


Wolbachia presence No
Confidence level High
Explanation of confidence level

16s rRNA gene of Wolbachia did not show up in Gel Electrophoresis after PCR; as a band of DNA. This shows us that the insect Isoptera that I collected did not contain Wolbachia DNA; thus, the sample is Wolbachia negative.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isoptera was found to be negative for Wolbachia.
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