Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/23/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | -under old debris laying on ground -dirt and sand, damp, dark, place with wood pieces |
| Putative identification | Arthropoda Hexapoda Insecta Isoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | 16s rRNA gene of Wolbachia did not show up in Gel Electrophoresis after PCR; as a band of DNA. This shows us that the insect Isoptera that I collected did not contain Wolbachia DNA; thus, the sample is Wolbachia negative. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Isoptera was found to be negative for Wolbachia. |
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1
Oniscus asellus GC1
Oniscus Asellus (LS1)