GRP2 Coccinellidae #1

May 15, 2024

Sample information
Picture
Photos by: Kayla J.
Location
Collection date 05/13/2024
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

The bug was found in a dry, sparse environment. Crawling in green weeds. Dirt and not too much plants growing around the area. The temperature was around 64º-68ºF.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Coccinellidae

Methods
Extraction kit tweezers
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coccinellidae was found to be postive for Wolbachia.
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